Selection of <i>Pichia pastoris</i> clones expressing hS1P<sub>1</sub> and receptor purification.
- Franck Talmont (305344)
- Lionel Moulédous (305345)
- Marion Baranger (6433385)
- Anne Gomez-Brouchet (6433388)
- Jean-Marie Zajac (6433391)
- Clarence Deffaud (6433394)
- Olivier Cuvillier (256709)
- Anastassia Hatzoglou (3420)
Publication date
March 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
A Expression of hS1P1 in different transfected Pichia pastoris clones (1 to 10). B (a) Purified hS1P1 eluted with 300 mM imidazole. (b) Expression of hS1P1 in crude supernatant from Pichia pastoris after cell breakage and centrifugation at 1,000g. Detection of receptors were realized after SDS-PAGE and Western blotting using an anti-cmyc antibody (1:1,000, v/v).</p
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