Fluorescence micrographs of HBEC-5i cells stained with FITC phalloidin for F-actin detection. Note that CNF1, but not CNF1 C866S, induce a remarkable reorganization of actin in stress fibers and membrane protrusions and that these modifications are partially reduced when cells are pre-treated with the Rock inhibitor Y27632 and the Rac/Cdc42 inhibitor ML141. Bar = 10 μm.</p
Rearrangements of the actin cytoskeleton underlie many physiological processes. I investigated actin...
<p>(A) HKC-8 cells were transfected with siRNAs as indicated. After 48 h the cells were stimulated w...
<p>A) HeLa cells non-transfected, or transfected with control or Net1 specific shRNA or siRNA, were ...
(A) SEM analysis showing HBEC-5i cells activated with TNF-α (control) and TNF-α-activated cells trea...
Cytotoxic necrotizing factor 1 (CNF1), a 110-kDa protein toxin from pathogenic Escherichia coli indu...
<p>Treatment with a cGMP agonist (8-Br-cGMP, 1 μM) or PKG agonist (8-pCPT-cGMP, 1 μM) alone was able...
The dynamics of the actin cytoskeleton and its connection to endothelial cell–cell junct...
<p>Neurons were either continuously exposed to CNF1 until DIV 21, or exposed to CNF1 until DIV 9 and...
<p> Cells were treated with TNF-α (B) for 2 hours or with O-Me-cAMP (C) for 1 hour or co-treated wit...
<p>(<b>A</b>) J774A.1 macrophages were treated with 25 nM purified CNF<sub>Y</sub> toxin for 3 h. To...
<p>Cytoskeletal organization and Rac1 activity in endothelial monolayers is altered by simvastatin t...
<p>(A) Confocal micrographs of CM and TCM-treated MSCs (a–c) and 3T3 fibroblasts (d–e) stained with ...
<p>(<b>A</b>) Localization of ARHGEF11 in stably transduced NRK cells [-LVC (lentiviral scramble con...
<p>(A) Cos-7 cells were transfected with GFP (control) or GFP-NESH, after which the F-actin was stai...
<p>A) Immunoblot analysis of the effects of H-1152 (top) or Y-27632 (bottom) concentration on induct...
Rearrangements of the actin cytoskeleton underlie many physiological processes. I investigated actin...
<p>(A) HKC-8 cells were transfected with siRNAs as indicated. After 48 h the cells were stimulated w...
<p>A) HeLa cells non-transfected, or transfected with control or Net1 specific shRNA or siRNA, were ...
(A) SEM analysis showing HBEC-5i cells activated with TNF-α (control) and TNF-α-activated cells trea...
Cytotoxic necrotizing factor 1 (CNF1), a 110-kDa protein toxin from pathogenic Escherichia coli indu...
<p>Treatment with a cGMP agonist (8-Br-cGMP, 1 μM) or PKG agonist (8-pCPT-cGMP, 1 μM) alone was able...
The dynamics of the actin cytoskeleton and its connection to endothelial cell–cell junct...
<p>Neurons were either continuously exposed to CNF1 until DIV 21, or exposed to CNF1 until DIV 9 and...
<p> Cells were treated with TNF-α (B) for 2 hours or with O-Me-cAMP (C) for 1 hour or co-treated wit...
<p>(<b>A</b>) J774A.1 macrophages were treated with 25 nM purified CNF<sub>Y</sub> toxin for 3 h. To...
<p>Cytoskeletal organization and Rac1 activity in endothelial monolayers is altered by simvastatin t...
<p>(A) Confocal micrographs of CM and TCM-treated MSCs (a–c) and 3T3 fibroblasts (d–e) stained with ...
<p>(<b>A</b>) Localization of ARHGEF11 in stably transduced NRK cells [-LVC (lentiviral scramble con...
<p>(A) Cos-7 cells were transfected with GFP (control) or GFP-NESH, after which the F-actin was stai...
<p>A) Immunoblot analysis of the effects of H-1152 (top) or Y-27632 (bottom) concentration on induct...
Rearrangements of the actin cytoskeleton underlie many physiological processes. I investigated actin...
<p>(A) HKC-8 cells were transfected with siRNAs as indicated. After 48 h the cells were stimulated w...
<p>A) HeLa cells non-transfected, or transfected with control or Net1 specific shRNA or siRNA, were ...