The IHC studies for BBB junctional proteins occludin and claudin-5.

IHC studies showing significant increases in occludin and claudin-5 expressions in the brain meninges in the second and third weeks after infection compared to the controls and those infected for 1 week. Dexamethasone administration significantly decreased the expressions of occludin and claudin-5 in the mice meninges. Scale bar, 10 um at 10x and 40x magnification. Control: no parasitic infection; 1W: 1 week after infection; 2W: 2 weeks after infection; 3W: 3 weeks after infection; 3W + Dex: mice given dexamethasone for 2 weeks (7th to 21st day post infection) and sacrificed on day 21.