Maternal resistin increases offspring hypothalamic inflammation and reactive gliosis markers.

Total mRNA prepared from the hypothalamus of dams and offspring (p28 and p90) were used to determine gene expression of different markers using RT-qPCR. Gene expression was normalized to GAPDH and 18S. Hypothalamic inflammatory markers are presented in panel A for dams, panel D for p28 offspring and panel G for p90 offspring. Hypothalamic gliosis markers are presented in panel B for dams, in panel E for p28 offspring and panel H for p90 offspring. Hypothalamic insulin/leptin responsiveness markers are presented in panel C for dams, panel F for p28 offspring and panel I for p90 offspring. Data are presented as boxplots (n = 4–5). *P<0.05 when comparing CC group to CR group using non-parametric Mann & Whitney statistical test.