Ag85B-BCG-CWS-treated DCs stimulate T cells to produce Th1 and Th17 cytokines.

T cells were purified from splenocytes of naive mice via MACS column. The T cells were co-cultured with unstimulated DCs, Ag85B (2.5 μg/ml)-stimulated DCs, MPL (5 μg/ml)-stimulated DC, MPL (5 μg/ml)+Ag85B (2.5 μg/ml)-stimulated, BCG-CWS (5 μg/ml)-stimulated DCs, or Ag85B (2.5 μg/ml)-BCG-CWS(5 μg/ml)-stimulated DCs for 5 days at a DC to T cell ratio of 1:10. The cytokine levels in the culture supernatants were measured by an ELISA. The data shown are the mean values ± SD (n = 3); *p p p < 0.001, compared to control group.