The long-term protective potential of Ag85B-BCG-CWS against Mtb challenge.

(A) Schematic diagram of the experimental design. Eight weeks after the first vaccine injection, mice were challenged with virulent Mtb. (B) Six or (C) thirty-two weeks post-challenge, the mice were sacrificed and the bacterial burden (CFU) was measured in the lung and spleen. The results from one of the two experiments producing similar results are shown (n = 3 animals/group). *p p p < 0.001 compared to each to group 1. Group G1: Infection control, G2: BCG alone, G3: Heat-killed BCG, G4: BCG-CWS alone, and G5: Ag85B-BCG-CWS.