Validation of RNAseq results in DIO alpha-cell through real-time quantitative PCR analyses.

FACS-sorted Venus+ alpha cells from control LFD (black bars) and obese hyperglycemic HFD (grey bars) mice were collected and analysed for mRNA quantification of altered genes identified by RNAseq. The measurement of different mRNA levels of target genes was performed by real-time PCR (Light-cycler LC480). Data are relative to three housekeeping genes (Rps9, Hprt and Ppia) and presented as the means (Fold of controls) ± SEM (n = 7 mice for LFD, n = 6 for HFD group). * means significant compared to LFD.