Additional file 1 of Circulating tumor cell investigation in breast cancer patient-derived xenograft models by automated immunofluorescence staining, image acquisition, and single cell retrieval and analysis
- Arturo Ramirez (6468611)
- Raksha Bhat (6468614)
- Debashish Sahay (564139)
- Carmine De Angelis (2872475)
- Hariprasad Thangavel (6468617)
- Sina Hedayatpour (6468620)
- Lacey Dobrolecki (3578129)
- Agostina Nardone (6468623)
- Mario Giuliano (6468626)
- Chandandeep Nagi (286802)
- Mothaffar Rimawi (6468629)
- C. Osborne (6468632)
- Michael Lewis (339161)
- Jackie Stilwell (2660011)
- Eric Kaldjian (6468635)
- Rachel Schiff (27253)
- Meghana Trivedi (6468638)
Publication date
March 2019
DOIAbstract
Figure S1. Linearity of spike-in CTC counts at low blood volumes. SK-BR-3 breast cancer cells were spiked into control mouse blood at approximately 500 cells/mL. Aliquots of 1000, 500, 250, 125 and 62.5 ΟL were made and processed according to the mouse blood protocol. Slides were analyzed after staining and imaging. CTC counts throughout the range of volumes tested were highly linear (R2 = 0.97). (PPTX 37 kb
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