G9a is required for resistance to paraquat-induced OS.

(A) Survival curves of G9a-null mutants and controls upon paraquat-induced OS exposure (treated) show reduced survival in G9aDD1 mutants (median survival time: G9aDD1 mutants, treated: 24 h, n = 324; versus control, treated, 48 h, n = 330; p G9aDD1, untreated n = 632; control, untreated n = 697). (B) Survival curves of G9a ubiquitous knockdown flies show reduced survival upon OS exposure (actin-Gal4 + UAS-G9a-RNAi, treated) compared to the controls (actin-Gal4, treated) (median survival time: actin-Gal4 + UAS-G9a-RNAi, treated, 14 h, n = 48 versus actin-Gal4, treated, 18 h, n = 48; p = 0.007) and normal longevity when untreated. The ubiquitously expressed actin driver was combined with a UAS-G9a RNAi construct (actin-Gal4 + UAS-G9a-RNAi) to knock down G9a expression or crossed to the isogenic background of the RNAi construct to generate the isogenic control (actin-Gal4). Survival curves showing percent survival over time and SE were plotted using Graphpad, and p-values were obtained using the Gehan-Breslow-Wilcoxon statistical test. All experiments were independently replicated at least three times. The numerical data depicted in this figure can be found in S5 Data. OS, oxidative stress; RNAi, RNA interference; SE, standard error.