MOESM4 of In vivo compartmental kinetics of Plasmodium falciparum histidine-rich protein II in the blood of humans and in BALB/c mice infected with a transgenic Plasmodium berghei parasite expressing histidine-rich protein II

Additional file 4: Figure S3. Investigation of the presence of once-infected RBCs pre-treatment. iRBCs were isolated from a P. falciparum infected patient with a parasitaemia of 2.9% quantified by blood film prior to receiving treatment. No once-infected RBCs were observed prior to treatment. iRBCs were defined as cells with positive PfHRP2 (red) and DAPI staining (blue). Once-infected RBCs were defined as cells with positive PfHRP2 staining (red) but no DAPI staining (blue)