Eukaryotic DNA binding proteins must access genomic DNA that is packaged into chromatin in vivo. During a productive infection, retroviral integrases (IN) must similarly interact with chromatin to integrate the viral cDNA genome. Here we examine the role of nucleosome DNA unwrapping in the retroviral integrase search for a target site. These studies utilized PFV intasomes that are comprised of a tetramer of PFV IN with two oligomers mimicking the viral cDNA ends. Modified recombinant human histones were used to generate nucleosomes with increased unwrapping rates at different DNA regions. These modifications included the acetylmimetic H3(K56Q) and the chemically engineered H4(K77ac, K79ac). While transcription factors and DNA damage sensors...
To establish productive infection, a retrovirus must insert a DNA replica of its genome into host ce...
<div><p>Retroviral integrases (INs) catalyse the integration of the reverse transcribed viral DNA in...
International audienceChromatin regulates the selectivity of retroviral integration into the genome ...
Eukaryotic DNA binding proteins must access genomic DNA that is packaged into chromatin in vivo. Dur...
Retroviral integration is catalysed by a tetramer of integrase (IN) assembled on viral DNA ends in a...
Retroviral integrase can efficiently utilise nucleosomes for insertion of the reverse-transcribed vi...
International audienceBACKGROUND:Retroviral integration depends on the interaction between intasomes...
Background: Stable insertion of the retroviral DNA genome into host chromatin requires the functiona...
Integration of viral DNA into the host chromosome is an obligatory step in retroviral replication an...
Integration of viral DNA into the host chromosome is an obligatory step in retroviral replication an...
The integration of the retroviral genome into the chromatin of the infected cell is catalysed by the...
To establish productive infection, a retrovirus must insert a DNA replica of its genome into host ce...
<div><p>Retroviral integrases (INs) catalyse the integration of the reverse transcribed viral DNA in...
International audienceChromatin regulates the selectivity of retroviral integration into the genome ...
Eukaryotic DNA binding proteins must access genomic DNA that is packaged into chromatin in vivo. Dur...
Retroviral integration is catalysed by a tetramer of integrase (IN) assembled on viral DNA ends in a...
Retroviral integrase can efficiently utilise nucleosomes for insertion of the reverse-transcribed vi...
International audienceBACKGROUND:Retroviral integration depends on the interaction between intasomes...
Background: Stable insertion of the retroviral DNA genome into host chromatin requires the functiona...
Integration of viral DNA into the host chromosome is an obligatory step in retroviral replication an...
Integration of viral DNA into the host chromosome is an obligatory step in retroviral replication an...
The integration of the retroviral genome into the chromatin of the infected cell is catalysed by the...
To establish productive infection, a retrovirus must insert a DNA replica of its genome into host ce...
<div><p>Retroviral integrases (INs) catalyse the integration of the reverse transcribed viral DNA in...
International audienceChromatin regulates the selectivity of retroviral integration into the genome ...