MOESM8 of DNA replication dynamics of vole genome and its epigenetic regulation

Additional file 9. DNA content analysis independent of S-phase pattern staging. The data shown in Figure 7A were binned using the DNA content of the total cell population (A) to test for user-dependent S-phase pattern classification biases. Then, the frequency of the S-phase patterns assigned was plotted per DNA content bin. The early (red), mid (blue) and late (green) S-phase patterns corresponded well to the progression of genomic DNA content increase during S-phase in untreated cells (B). In contrast, in LBH-treated cells the distribution of the early replication pattern occurred within cells with a higher DNA content (red), while the late replication pattern appeared unaffected