Effect of chronic administration of LIMK2 inhibitors on the content of fibroblasts positive for phosphorylated Cofilin after 30 days of surgery.

(A) Representative images for double immunofluorescent staining of cavernosal tissue with anti-Vimentin and anti-phospho-Cofilin in the S, I and L groups using confocal microscope. White arrows indicate significant expression of phosphorylated Cofilin in cavernosal fibroblasts (yellow color in merged or magnified image). Scale bar = 100 μm. (B) A bar graph showing the comparison in fibroblasts positive for phosphorylated Cofilin (mean ± SEM) among the S, I and L groups. S = sham surgery group, I = bilateral CNCI group, L = bilateral CNCI group treated with chronic administration of LIMK2 inhibitors. * p < 0.05 vs. I group; † p < 0.05 vs. S group.