Physical interaction between Jab1 and the sW182* truncation mutant, but not wild-type LHBs.
- Shu-Yi Chiu (6459119)
- Hsiang-Ju Chung (6459122)
- Ya-Ting Chen (442977)
- Min-Syuan Huang (6459125)
- Chien-Chih Huang (757880)
- Shiu-Feng Huang (442981)
- Isao Matsuura (526707)
Publication date
March 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
Upper panel: Flag-tagged wild-type LHBs or sW182* mutant expressed in HK293T cells was pulled down with GST-Jab1-beads. The precipitated proteins were subjected to Western blot using Flag antibody. Vector control was also included. Lysate (6%) used for the assay is shown as Input. The precipitated sW182* protein is indicated by an arrowhead. Asterisks indicate nonspecific bands in the precipitate. Lower panel: Flag-tagged Jab1 and 6xHis-Myc-tagged wild-type LHBs or sW182* mutant were co-expressed in HK293T cells. Myc-tagged protein was precipitated by Myc antibody-conjugated agarose beads and Jab1 was detected by Flag antibody. Lysate (1.5%) used for the assay is shown as Input.</p
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