Basal medium with autologous plasma (AP) or autologous platelet lysate (APL) results in loss of cell surface marker phenotype and impaired growth respectively.

Flow cytometry analysis shows lack of CD90 expression by MSC cultured for 4 days in basal medium + AP (B) compared to MSCs cultured in basal medium + FBS (A) and compared to MSCs cultured in basal medium + APL (C). (D): MSC cultured for 4 days in basal medium + FBS, (E): MSC cultured for 4 days in basal medium + APL. (F): concentration of harvested cells was determined 4 days after the cells were plated and shows a decrease in the growth rate when the cells were cultured in basal medium + APL compared to cells cultured in basal medium + FBS (n = 2). Abbreviations: MSC, mesenchymal stem cell; FBS: fetal bovine serum.