Mouse MSCs inhibit T-cell proliferation via NO production and induction of cellular stress response.
- Adam G. Laing (6459506)
- Giorgia Fanelli (124917)
- Andrei Ramirez-Valdez (6459509)
- Robert I. Lechler (201029)
- Giovanna Lombardi (139439)
- Paul T. Sharpe (10768)
Publication date
March 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) Proliferation of αCD3/CD28-activated CFSE-labelled CD4+ T-cells cultured in direct contact with mMSC ± iNOS inhibitor LNMMA (n = 4). (B) Representative immunoblot and (C) quantification of GCN2 phosphorylation (pGCN2) in αCD3/CD28-activated CD4+ T-cells co-cultured with mMSC or mMSC in the presence of L-Arg. (D) Proliferation of αCD3/CD28-activated CFSE-labelled CD4+ T-cells cultured in 100% non-activated or IFN-γ/TNF-α activated mMSC-conditioned medium.</p
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