Additional file 9: of MCT-1/miR-34a/IL-6/IL-6R signaling axis promotes EMT progression, cancer stemness and M2 macrophage polarization in triple-negative breast cancer
- Yueh-Shan Weng (6477854)
- Hong-Yu Tseng (6477857)
- Yen-An Chen (6477860)
- Pei-Chun Shen (6477863)
- Aushia Haq (6477866)
- Li-Mei Chen (47129)
- Yi-Chung Tung (263328)
- Hsin-Ling Hsu (169102)
Publication date
March 2019
DOIAbstract
Supplementary Methods. Cell culture. Plasmid construction and transfection. Targeting MCT-1 gene. Targeting IL-6 gene. Antibodies (Abs) and protein analysis. MCF-10A acinar morphogenesis. Cell invasion and migration assays. Gelatin zymography assay. Cell fractionation. Quantitative RT-PCR analysis of cancer stemness markers. ALDEFLUOR assay. Immunohistochemistry study. Quantification of miR-34a levels. Statistical analysis. (PDF 227 kb
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