MOESM1 of Antibacterial and anticancer activities of orphan biosynthetic gene clusters from Atlantis II Red Sea brine pool

Additional file 1: Figure S1. Anti-bacterial overlay assay results on 96-well plates. Zones of inhibition of 14-7E (a), 10-2G (b1) and E. coli CBAA11 (positive control) (b2), against Bacillus sp. Cc6. A single petri dish contained 96 clones. Figure S2. Sequence maps of all the putative PEGs on 14-7E insert. Dark blue: regulatory genes, purple: resistance genes, pink: NRPSs and PKSs, Green: transferases and synthases, orange: hydrolases and peptidases, dark red: Endonucleases, blue: ORFs with significant hits, grey: ORFs with non-significant hits yellow: tRNA-Met-CAT and magenta: radical SAM domain proteins and class I SAM-dependent methyltransferases. Figure S3. Cell viability percentage of cell lines after exposure to selected whole cell lysates. (A) MCF-7 cells, (B) U2OS cells and (C) 1BR hTERT cells, after 48 h exposure to extracts of: 14-7E (red) and 10-2G (green). Also presented are the media controls (dark blue) and 50% v/v buffer controls (light blue). The x-axis indicates the concentrations of the whole cell extracts (%v/v). The presented data for each condition is the mean of at least three independent experiments. P values are denoted as follows: & ≤ 0.05, # ≤ 0.01 and § ≤ 0.001. Figure S4. Phylogenetic trees as predicted by MEGAN for the insert DNA of (a) 14-7E and (b) 10-2G. Figure S5. Representative photos of MCF-7 (A) and 1BR hTERT (B) cells after exposure to 14-7E lysates for 48 h (200× magnification)