Effect of FleQ binding sites mutation on Pl<i>apA</i> expression.

β-galactosidase assays of the PlapA promoter fusion and FleQ-2 and FleQ-3 mutated promoters fusions in the wild-type strain KT2442 and ΔfleQ mutant (MRB52). Bars represent the averages and standard deviations of three independent assays. Stars designate p-values for the Student's t-test for unpaired samples not assuming equal variance. *: p<0.05, **: p<0.01; ***: p<0.001.