Gel mobility shift assays on the P<i>lapA</i> and P<i>bcsD</i> promoters.

Panel A: Cartoon of the probes used for each of the promoter regions, indicating the sizes (in bp) of the resulting fragments and the location of the predicted FleQ binding motifs (open boxes). Drawn to scale. Panels B, C and D: Autoradiograph of a representative PAGE gel containing the indicated probe, 0, 1 or 2 μM FleQ and 0, 1 or 2 μM FleN. Assays were performed in the absence (-) or in the presence (+) of c-di-GMP and in the absence (-) or in the presence (+) of ATP. Closed arrowheads denote the free DNA probes and open arrowheads denote the retarded complexes.