Agarose gel visualization of RT-PCR 113 bp products.

Amplification of Zika RNA extracted from cell lysates from the target cell lines, at days 1,2,3,6, and 8 of infection. Positive strand products were amplified using the negative primer and negative strand products using the positive primer in the initial reverse transcription (RT) step. Product size is indicated by a 100 base pair ladder. To verify that no products were present without the initial RT step, representative HepG2 cell lysates were amplified with and without the reverse transcription enzyme. No bands appear in the lanes which had no RT enzyme. Positive strand virus with RT is more abundant than negative strand virus.