Altered PGES2 levels.

(A) Western blotting of PGES2 protein expression from FACS-isolated astrocytes were imaged and quantified for IRloxp and IRKOGFAP mice. IRloxp (black bar) and IRKOGFAP (white bar), (n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. *P GFAP versus IRloxp group. The underlying data can be found in S1 Data. (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKOGFAP, astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.