Gene expression changes in Cdkn1b-/- mammary epithelial cells.

Lina Ding (218660)
Lauren B. Shunkwiler (6490868)
Nicholas W. Harper (6490871)
Yang Zhao (39052)
Kunihiko Hinohara (6490874)
Sung Jin Huh (2401033)
Muhammad B. Ekram (204699)
Jan Guz (6490877)
Michael J. Kern (6490880)
Alexander Awgulewitsch (6490883)
James D. Shull (693409)
Bart M. G. Smits (342237)
Kornelia Polyak (87725)

Open link

Publication date

April 2019

DOI

10.1371/journal.pgen.1008002.g003

Publisher

Public Library of Science (PLoS)

Abstract

(A) PCA plot of luminal (Cd24+Cd29low) and luminal progenitor (Cd24+Cd29lowCd49b+ and Cd24+Cd29lowPNA+) cells. (B) Numbers of significantly up and down regulated genes in Cdkn1b-/- rats. MEC (all mammary epithelial cells), BAS (basal), LUM (luminal), LP (luminal progenitor). (C) Top enriched process networks (FDR Cdkn1b+/+ and Cdkn1b-/- mammary glands. Scale bar 75 μM. (E) Quantification of the fractions of FoxA1+ and Pr+ cells. Statistical significance determined using Welch two sample t test of arcsin transformed values. (F) Immunoblot analysis of pStat5 in Cdkn1b+/+ and Cdkn1b-/- mammary epithelium. (G) Top enriched process networks (FDR < 0.1) in the indicated overlaps. (H) Top enriched process networks (FDR < 0.1) in basal and luminal ...