Additional file 1: of The miR155HG/miR-185/ANXA2 loop contributes to glioblastoma growth and progression

Figure S1. (A) U87 cells pretreated with a lentivirus with sh-miR155HG or sh-NC and a lentivirus containing luciferase were implanted in the brains of nude mice, and tumor formation was assessed by bioluminescence imaging. The bioluminescent images were measured at days 1, 11 and 21 after implantation. (B) Overall survival from two groups of nude mice injected with U87 cells, transfected with sh-NC or sh-miR155HG lentivirus, was determined by Kaplan-Meier survival curves, and a log-rank test was used to assess the statistical significance of the differences. (C) Expression levels of ANXA2 in GBM tissues and adjacent normal brain tissues were analyzed by western blotting and normalized to β-catenin. (D) The protein expression levels of ANXA2 were analyzed by western blotting after 48 h transfection in U87 and GP1 cells with scramble, miR155HG siRNA 1, miR155HG siRNA 2, miR-NC or inhibitor, respectively. (E) The effect of sh-ANXA2 in U87 cell and tumor tissue of nude mice after implantation were analyzed by western blotting. (E) Downregulating ANXA2 contributed to the reduction of p-STAT3 level in GBM cells. (TIF 9482 kb