Inflammatory monocytes promote detrimental host responses to <i>Cryptococcus neoformans</i>.

(A) IM in the lungs of C57BL/6 (WT) mice after intratracheal (i.t.) challenge with C. neoformans strain H99 relative to naive mice (dotted line). Data were pooled from eight independent experiments (n = 6–26 total mice per timepoint). (B) Diphtheria toxin (DT) was administered intraperitoneally (i.p.) as illustrated to ablate IM in CCR2-DTR mice. (C) Kaplan-Meier survival curve of WT littermate controls (white circles) and CCR2-DTR mice (black circles) after administration of DT and H99. Data were pooled from two independent experiments (n = 10 total mice per group). (D-F) CFU in (D) lung, (E) mediastinal lymph node (LN), and (F) brain homogenates from WT and CCR2-DTR mice at indicated timepoints. Data were pooled from nine independent experiments (n = 7–27 total mice per group per timepoint). *, P P P P < 0.0001.