Gene expression profile of before and after astrocyte differentiation (day 30).

(A) Before and after gene expression of NPC specific markers: SOX2, MSI1, and NES. WT and shHD showed a significant decrease in SOX2 and NES expression. shHD showed a significant decrease in MSI1 expression. (B) Before and after gene expression of astrocyte-specific markers: APOE, GFAP, GLT1, GRM1, and LCN2. All three cell lines showed a significant increase in GFAP, GLT1, and LNC2 expression. HD and shHD showed a significant increase in APOE expression while only WT showed a significant increase in GRM1 expression. (C) Mature astrocyte-specific marker expressions before and after the differentiation. All three cell lines showed significant increase in GFAP expression (WT P = 0.002, HD P = 0.003, shHD P FZD1 (P = 0.012, P S100β (P = 0.006, P 0.001). HD showed significant decrease in GRIA2 (P = 0.001), S100β (P = 0.008), and SERPINA3 (P SERPINA3 (P = 0.016). At least two biological replicates and three technical replicates were analyzed for this study. Statistical significance was determined by ANOVA (asterisks denote following * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001).