Impact of Uls1 on nucleosomes in the rDNA.

(A) Integrated genome viewer (igv) snapshot displaying the rDNA locus (two repeats). Nucleosome occupancies in WT, sir2 and uls1 strains plotted as blue peaks in the three middle tracks. Nucleosome differential signals between uls1-WT and sir2-WT are the top two tracks. Negative values represent lower nucleosome occupancy in the mutant strains and vice versa. Note that the differential tracks are statistical representations (-log10 (P-value) of Poisson tests, [35]) of the nucleosome differences and that the scales are different. Bottom tracks show the rDNA genes and the amplicons used to determine rDNA copy number. (B) The results of quantitative PCR using four sets of primers amplifying rDNA. Plotted is the average uls1/WT ratio for the E-PRO, ARS, COD and ENH amplicons from two independent experiments, with the variation indicated as error bars. ACT1 was used for normalizing.