F-actin visualization.

(A) Cells were left under basal condition (CTR) or treated with OLE (0.01 mg/ml), CdCl2(0.1μM) or with OLE in the presence of CdCl2. Specimens were incubated with Phalloidin-TRITC (400 μg/ml) for 45 min at room temperature to detect F-actin. (B) F-actin content was semi-quantified by actin polymerization assay. Confluent cells were treated as described in Methods. After staining with TRITC-phalloidin, cells were extracted with cold methanol and the fluorescence absorbance of extracts was read (540/565 nm) in an RF-5301PC fluorimeter. The values obtained were compared using one-way Anova and Tukey’s multiple comparison test (*P<0,05 vs CTR).