Vertebrate odorant binding proteins as antimicrobial humoral components of innate immunity for pathogenic microorganisms - Fig 1

Investigation of the binding of 1-aminoanthracene (A and C) and farnesol (B and D) to bovine (A) and porcine (B) OBP tagged with six histidine residues. The graphs report either the direct binding (A and C), or the chasing of the OBP fluorescent ligand 1-aminoanthracene in response of increasing concentrations of farnesol (B and D). The amount of 1-aminoanthracene bound to OBP is expressed on the y axis as arbitrary units of emission fluorescence intensity. The four graphs report the results of single experiments. The curves were treated either as hyperbolic increases (A and C) or decays (B and D), with r values higher than 0.97. (The original data of the graphs are reported in S1 Available data).