Exposure of hydrophobic patches at intermediate state of unfolded MsEis analyzed by ANS fluorescence.
- Shashi Anand (6504245)
- Arsheed Ahmad Ganaie (6504248)
- Charu Sharma (339391)
Publication date
March 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
Changes in ANS emission spectra (ʎmax = 518 nm) of MsEis protein in 20 mM phosphate buffer (pH 8.0) as a function of GdmCl (Panel A), urea (Panel B) and temperature (Panel C). Acquisition of ANS emission spectra was performed at 25°C, strictly in dark after incubating protein sample and ANS dye in 1:5 molar ratios for 5 min.</p
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