Comparison of MSD assays and Seprion ELISA.
- Wolfgang Reindl (5630)
- Barbara Baldo (500513)
- Jana Schulz (4183195)
- Isabell Janack (6508679)
- Ilka Lindner (6508682)
- Markus Kleinschmidt (6508685)
- Yalda Sedaghat (185223)
- Christina Thiede (139375)
- Karsten Tillack (720423)
- Christina Schmidt (36805)
- Isabell Cardaun (1655848)
- Tom Schwagarus (6508688)
- Frank Herrmann (218163)
- Madlen Hotze (6508691)
- Georgina F. Osborne (488792)
- Simone Herrmann (6508694)
- Andreas Weiss (135692)
- Celina Zerbinatti (275425)
- Gillian P. Bates (135712)
- Jonathan Bard (58130)
- Ignacio Munoz-Sanjuan (112471)
- Douglas Macdonald (562225)
Publication date
March 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
Homogenates of (A) cortex, (B) striatum, and (C) musculus tibialis anterior tissues of an aged series of R6/2 mice were analyzed at 1 mg/mL (cortex, TA) or 0.75 mg/mL (striatum) in the MW8 / 4C9-ST and MW8 / MW8-ST MSD assays, or using 15 μL of a 2.5% (w/v) ribolysate in the Seprion ELISA assay utilizing antibodies S830 or MW8. The background signal in the Seprion ELISA samples is generated by the substrate (see WT samples). Error bars represent standard deviations of four (WT) or eight (R6/2) biological replicates.</p
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