Specificity of the SmMIT-LAMP assay in simulated human urine samples spiked with DNA from several helminths.
- Pedro Fernández-Soto (403075)
- Javier Gandasegui (778298)
- Cristina Carranza Rodríguez (403077)
- José Luis Pérez-Arellano (403078)
- Beatriz Crego-Vicente (6508832)
- Juan García-Bernalt Diego (4567348)
- Julio López-Abán (134057)
- Belén Vicente (615664)
- Antonio Muro (134062)
Publication date
March 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
Figure shows the LAMP results when incubating for 60 min (top, by color change; bottom, by agarose electrophoresis) in urine samples spiked with S. mansoni DNA alone and in combination with DNA from other helminths. Samples were prepared, each containing 100 μL of urine spiked with 0.5 ng of DNA from each parasite. Sm, S. mansoni; Sh, S. haematobium; Si, S. intercalatum; Sb, S. bovis; Sj, S. japonicum; Fh, Fasciola hepatica; Amp, Amphimerus sp.; Sv, Strongyloides venezuelensis.</p
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