A live-animal sensor for lysosomal membrane integrity.
- Brian J. Thomas (6508961)
- Ira E. Wight (6508964)
- Wendy Y. Y. Chou (6508967)
- Marco Moreno (6508970)
- Zachary Dawson (6508973)
- Arielle Homayouni (6508976)
- Huiyan Huang (4927240)
- Hyori Kim (819938)
- Hanna Jia (6508979)
- Justin R. Buland (6508982)
- Jennifer A. Wambach (6508985)
- F. Sessions Cole (2311990)
- Stephen C. Pak (154777)
- Gary A. Silverman (154780)
- Cliff J. Luke (154783)
Publication date
March 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
Pnhx-2lmp-1::CemOrange2 transgenic C. elegans were exposed to either PBS (A, C, E, G) or 5% tert-butyl hydroperoxide (t-BOOH; B and D) in PBS for 30 min or 500 mM LLOMe (F and H) in PBS for 30 min. Animals were visualized by confocal microscopy using a 40x PlanApo oil immersion objective (N.A.1.3) at Ex555 nm/Em565-590 nm over >20 z-planes. Representative confocal images of animals shown as a single XY plane. Transgenic C. elegans exposed to PBS alone (A, C, E, G) have punctate LMP-1::CemOrange2 expression (A and E, red). The same animals exposed to 5% t-BOOH (B) or 500 mM LLoMe (F) lose punctate LMP-1::CemOrange2 labeled structures. DIC images (C, D, G, H) displayed for reference. Scale bars = 25 μm.</p
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