Additional file 6: of Network approach identifies Pacer as an autophagy protein involved in ALS pathogenesis

Figure S3. Pacer mRNA levels in the lumbar spinal cord from sALS patients and fALS mouse models. a, Human Pacer (hPacer) and b, human Rubicon (hRubicon) mRNA expression was determined by qPCR in postmortem spinal cord sections from sALS patients and age-matched control subjects. Left panel, cervical spinal cord section with Controls n=2 and sALS patients n=6; middle panel, thoracic spinal cord section with Controls n=2 and sALS patients n=7; and right panel, lumbar spinal cord section with Controls n=6 and sALS patients n=7. β-Actin mRNA levels were used for normalization. c, Pacer and Rubicon mRNA expression was determined by qPCR in lumbar spinal cord samples of late symptomatic TDP43A315T transgenic mice (TDP43A315T-Tg, n=5) and their non-transgenic littermate controls (n=3), respectively. β-Actin levels were used for normalization. d, Pacer and Rubicon mRNA expression was determined in the lumbar spinal cord of late symptomatic SOD1G93A transgenic mice (SOD1G93A-Tg) and their non-transgenic littermate controls (both groups, n=7). 18S RNA levels were used for normalization. (PPTX 362 kb