Additional file 1: of Functional networks are impaired by elevated tau-protein but reversible in a regulatable Alzheimer’s disease mouse model

Figure S1. Western blot results of tau expression in mice expressing pro- or anti-aggregant human tau repeat domain before and after switch-off. Lanes 1, 3: Pro-aggregant or anti-aggregant TauRD was expressed for ~ 12 months (ON), then analyzed by Western blotting. Lanes 2, 4: Pro- or anti-aggregant TauRD was expressed for ~ 12 months (ON), then switched off by doxycycline for 2 months (OFF). Hippocampal brain tissue was subjected to Western blotting for tau protein levels using antibody K9JA. In the absence of doxycycline (Tau-ON), the Western blots show human TauRD (Mr~ 14 kDa, pro-aggregant ON and anti-aggregant ON) and full-length mouse Tau (Mr~ 55 kDa) (lanes 1,3). After expression of pro- or anti-aggregant TauRD and then switching-off, the band of human TauRD has disappeared (lanes 2,4). Wildtype mice show only full-length mouse Tau (lane 5). (TIF 969 kb