Automated seeding overview.

(A) Example phase image. (B) First step of automated seeding algorithm: Pre-processing and watershed. In this step, the watershed transform is applied to the processed image. (C) Phase image with watershed lines (yellow). (D) Flowchart of the second step of automated seeding: Automated correction and fine-tuning. At this step, the cell boundaries are automatically fine-tuned, and segmentation errors are automatically corrected. (E) The result of the automated seeding step. Each cell boundary is marked with a different color.