A hypomorphic mutation in <i>gsp-2</i> results in transgenerational sterility phenotype.

(A) Incidence of males in gsp-2(yp14) was 3.9% at 20°C and increased to 16.8% at 25°C. When L1 animals were shifted to 25°C we saw a similar increase in males (5%, N = 42) to animals grown at 20°C and when L4 animals were shifted incidence of males was 10.7% (N = 49) (B) Progeny of gsp-2(yp14) animals grown at 20°C or 25°C were 6% and 41% Embryonic Lethal, respectively, compared to 97% of gsp-2(tm301) progeny (N = 20). (C-D) gsp-2(yp14) was identified to have a G to A mutation in exon 5 by whole genome sequencing. This results in a D to N amino acid substitution in a well conserved region of GSP-2. (E) When passaged at 20°C for many generations N2, gsp-2(yp14), lab-1 and lab-1; gsp-2(yp14) did not exhibit a loss of transgenerational fertility. (F) gsp-2(yp14) and lab-1 both exhibited loss of fertility at 25°C and were completely sterile by generation F17 and F11 respectively. A double mutant of lab-1;gsp-2 went sterile slightly faster than the individual single mutants and were completely sterile by F10. (N≥40) (G) Analysis of incidence of males showed no jackpots of males at in gsp-2(yp14) animals. *P <.0001 by T-test. Error bars represent standard deviation.