Changes in metabolites of calves underwent milk restriction or supplemented with prebiotics.

Serum levels of lactate (Fig 2A), protein (Fig 2B), triglycerides (Fig 2C), glucose (Fig 2D), creatinine (Fig 2E), urea (Fig 2F) and alkaline phosphatase (Fig 2G) of calves fed milk restricted or supplemented with prebiotics during period 1 (gray bars) and period 2 (black bars). Data are presented as mean ± the standard error of the mean (SEM). *P<0,05. CON–control calves that received 6 L of milk/day for 56 days. RES–calves received restriction milk (3 L/day) for 28 days (Period 1) followed by 6 L milk/day for 28 days (Period 2). MOS–calves supplemented with 5 g/day of mannanoligosaccharides and same diet of CON animals. MOS calves supplemented with 5 g/day of manan-fructooligosaccharides and same diet of CON animals.