Additional file 1: of Autophagy inhibition enhances PD-L1 expression in gastric cancer

Figure S1. Flow cytometry histograms for PD-L1 expression of 8 gastric cancer cell lines. PD-L1 was expressed on 7.6% of AGS cells, 32.4% of NCI-N87 and 2.4% of SGC7901 cells. Less than 1% of BGC823, HGC27, MGC803, MKN45, SNU1 cells were detected to express PD-L1. Figure S2. LC3B positive puncta (green) was determined by immunofluorescence in AGS and NCI-n87 cells exposed to autophagy inhibitors, bafilomycin A1 (Baf, 10 nM) and chloroquine (CQ, 32 μM) for 24 h. Scale bar, 50 μm. Figure S3. The effect of chloroquine on the expression of PDL1 in in vivo subcutaneous xenograft models. Figure S4. (A) The effect of pharmacological inhibitors of autopahgy on the protein levels of p65, p-p65, IκBα, p-IκBα, IKKα/β and p-IKKα/β was detected by Western blots in AGS cells. Figure S5. (A) The protein levels of PD-L1, STAT1, p65 and p-p65 were detected by Western blots in AGS and NCI-n87 cells treated with IFN-γ for 24 h. Figure S6. (A) Representative images of double staining for LC3 and PD-L1. (DOC 5030 kb