ENZ radiosensitizes xenograft tumors.

A) LNCaP xenografts in nude mice were treated by vehicle (q.d. for 20 consecutive days), vehicle (q.d. for 20 consecutive days) + 2 Gy q.d. for 5 consecutive days, ENZ (q.d. for 20 consecutive days), or ENZ (q.d. for 20 consecutive days) + 2 Gy (q.d. for 5 consecutive days) (N = 5 per treatment group). B &C) LNCaP xenograft immunohistochemical staining, quantified by automated cell counting using standard Ariol analysis scripts (Leica) for detecting positive cells (DAB stain). B) Percent cells positive for cleaved caspase-3 (apoptosis marker). Total cells counted: 1,504,915, 1,914,981, 675,835, and 379,511 for vehicle, ENZ, 2 Gy, and ENZ+2Gy, respectively.