MOESM1 of Citrullination of HP1γ chromodomain affects association with chromatin

Additional file 1: Figure S1. Citrullination of HP1γ identified by MS/MS. A Multiple sequence alignment of paralogues of mouse HP1 protein sequences using Clustal Omega. Functional domains of HP1 (CD, HR and CSD) and C-terminal and N-terminal extensions are colour-coded as indicated in panel D. Isoleucine residues in the P × V × L motif, known to diminish dimerisation of HP1, are highlighted in yellow. B/C MS/MS fragmentation tables relating to the fragmentation spectra depicted in Fig. 1B and C, including the mass accuracies of identified fragments. D Schematic illustration of HP1γ mutants used in this study. Changes in amino acids are indicated. Functional domains of HP1 are illustrated: CD (blue) is the chromodomain; HR (orange) is the hinge region; and CSD (green) is the chromoshadow domain. C-terminal and N-terminal extensions are indicated in gre