Knock down WTX promoted cervical cancer cell migration and invasion.

(A-B) WTX expression in SiHa cells transfected with siWTX-1#, 2#, 3# and siRNA-control. Changes in mRNA abundance were determined by qRT-PCR. Changes in protein abundance were determined by western blotting. (C-D) β-catenin expression in SiHa cells transfected with siWTX-2# and siRNA-control. Changes in mRNA abundance were determined by qRT-PCR. Changes in protein abundance were determined by western blotting. (E) Cell migration was analyzed using a wound-healing assay. The migration of SiHa transfected with siWTX-2# was increased, compared with that of cells transfected with siRNA-control. (F) Cell migration and invasion were analyzed using a Transwell assay. The migration and invasion of SiHa transfected with siWTX-2# was increased compared with that of cells transfected with siRNA-control. All values are presented as the means ± SEM, **P P P < 0.0001.