Additional file 8 of Expression of C-terminal ALK, RET, or ROS1 in lung cancer cells with or without fusion

Figure S4. Comparison of molecular weights between wild-type and fusion protein. After high exposure of N-terminal EML4 in the results shown in Fig. 2, two sizes of EML4 protein were detected in NCI-H2228 and SNU-2292 cell lines (left-hand figure), and we assumed that the larger protein was wild-type EML4 (arrow) and the smaller protein was ALK-fused EML4 (arrowhead), after referring to the protein weight of EML4-ALK detected by an ALK C-terminus antibody (right-hand figure) (a). In the same way, we assumed that the larger and smaller proteins were respectively wild-type EML4 (arrow) and ALK-fused EML4 (arrowhead) in the results for the ILS31007 tumor tissue specimen shown in Fig. 3b (b), and the larger and smaller proteins were respectively wild-type CCDC6 (arrow) and RET-fused CCDC6 (arrowhead) in the results for CCDC6 in the LC-2/ad cell line shown in Fig. 2 (c) (PPTX 600 kb