Additional file 3: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Additional file 2: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey MiannĂŠ (3407429)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S1. Screening by Sanger sequencing of animals for the generation of a Syt7 conditional allele...

Additional file 1: of Establishment of TP53-knockout canine cells using optimized CRIPSR/Cas9 vector system for canine cancer research

Kiyoung Eun (6173807)
Min Gi Park (6173810)
Yeon Woo Jeong (6173813)
Yeon Ik Jeong (6173816)
Sang-Hwan Hyun (295152)
Woo Suk Hwang (295153)
Sung-Hak Kim (2930070)
Hyunggee Kim (75727)

January 2019

Figure S1. Representative images of isolated canine fibroblasts with an extended cellular life span ...

Additional file 13: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey Mianné (3407417)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S12. Examples of unexpected point mutations in the F0 animals obtained from the co-injection ...

Additional file 2: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Ke Qin (1288881)
Xinyu Liang (487152)
Guanjie Sun (6553118)
Xuan Shi (739853)
Min Wang (21070)
Hongbo Liu (124714)
Yaosheng Chen (134488)
Xiaohong Liu (50816)
Zuyong He (134485)

April 2019

Figure S1. Fluorescent images of porcine kidney cells 24âh after transfection of plasmid pX458-sgR...

Additional file 4: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Ke Qin (1288881)
Xinyu Liang (487152)
Guanjie Sun (6553118)
Xuan Shi (739853)
Min Wang (21070)
Hongbo Liu (124714)
Yaosheng Chen (134488)
Xiaohong Liu (50816)
Zuyong He (134485)

April 2019

Figure S3. Images of cell clones expanded from single porcine kidney cell. One weekâs culture of s...

Additional file 6: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Ke Qin (1288881)
Xinyu Liang (487152)
Guanjie Sun (6553118)
Xuan Shi (739853)
Min Wang (21070)
Hongbo Liu (124714)
Yaosheng Chen (134488)
Xiaohong Liu (50816)
Zuyong He (134485)

April 2019

Figure S5. Further detection of the potential off-target effects of sgRNA17â6. NC indicates the ne...

Additional file 1: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Ke Qin (1288881)
Xinyu Liang (487152)
Guanjie Sun (6553118)
Xuan Shi (739853)
Min Wang (21070)
Hongbo Liu (124714)
Yaosheng Chen (134488)
Xiaohong Liu (50816)
Zuyong He (134485)

April 2019

Table S1. List of sgRNAs designed for targeting the intron16 and intron17 of KIT gene. Table S2. Pri...

Additional file 1: Table S1. of Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs

Jung-Taek Kang (3429896)
Bumrae Cho (304017)
Junghyun Ryu (3429890)
Caitlin Ray (3429908)
Eun-Jin Lee (2299186)
Yun-Jin Yun (3429902)
SunMi Ahn (3429899)
JinSeok Lee (3429905)
Dal-Young Ji (3429887)
Nathaniel Jue (3429884)
Sherrie Clark-Deener (550129)
Kiho Lee (1366734)
Kwang-Wook Park (3429893)

November 2016

Oligos used to introduce sgRNA into px330. Each pair of forward and reverse primers were annealing a...

Additional file 3: Figure S2. of One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system

Methichit Wattanapanitch (627973)
Nattaya Damkham (4889524)
Ponthip Potirat (4889521)
Kongtana Trakarnsanga (597732)
Montira Janan (4889527)
Yaowalak U-pratya (627977)
Pakpoom Kheolamai (627979)
Nuttha Klincumhom (627974)
Surapol Issaragrisil (627981)

February 2018

Showing transfection efficiency of PX458 in the Eβ-iPSC2 cells. (a) Phase contrast and fluorescent i...

Additional file 3: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Figure S1. showing sequencing results of parental and inserted iPSCs. a Parental iPSCs have the know...

Additional file 3: Figure S1. of Deciphering the genetic regulation of peripheral blood transcriptome in pigs through expression genome-wide association study and allele-specific expression analysis

T. Maroilley (4684477)
G. Lemonnier (2370415)
J. Lecardonnel (4684486)
D. Esquerré (4684474)
Y. Ramayo-Caldas (4684483)
M. Mercat (4684492)
C. Rogel-Gaillard (4684480)
J. Estellé (4684489)

December 2017

Distribution of eQTL-SNPs along pig autosomal chromosomes. The histograms represent the distribution...

Additional file 1: of Genome-wide identification of RNA editing in seven porcine tissues by matched DNA and RNA high-throughput sequencing

Yuebo Zhang (595294)
Longchao Zhang (465233)
Jingwei Yue (4439236)
Xia Wei (2063044)
Ligang Wang (347845)
Xin Liu (43569)
Hongmei Gao (240295)
Xinhua Hou (108350)
Fuping Zhao (389140)
Hua Yan (211621)
Lixian Wang (465239)

March 2019

Figure S1. Overview of the experimental design. Figure S2. Results of the Sanger sequencing validati...

Additional file 1: of Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells

Chenzi Zhang (6163493)
Xiangjun He (6163496)
Yvonne Kwok (6163499)
Feng Wang (44414)
Junyi Xue (1533826)
Hui Zhao (7395)
Kin Suen (6163502)
Chi Wang (131921)
Jianwei Ren (438694)
George Chen (179705)
Paul Lai (6163505)
Jiangchao Li (290762)
Yin Xia (307747)
Andrew Chan (352984)
Wai-Yee Chan (18789)
Bo Feng (141991)

December 2018

Figure S1. Cytogenetic analysis of the human cell line LO2. Figure S2. NHEJ-based knock-in of ires-G...

Additional file 1: of Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos

Kayla Carey (6670022)
Junghyun Ryu (3429890)
Kyungjun Uh (6670031)
Andrea Lengi (6670037)
Sherrie Clark-Deener (550129)
Benjamin Corl (6670040)
Kiho Lee (1366734)

May 2019

Table S1. Summary of off-targeting comparison for RAG2. Target gene (RAG2) guide sequence compared t...

Additional file 3: of In vivo genome and base editing of a human PCSK9 knock-in hypercholesterolemic mouse model

Alba Carreras (6210875)
Luna Pane (6163538)
Roberto Nitsch (6210878)
Katja Madeyski-Bengtson (4452094)
Michelle Porritt (5940611)
Pinar Akcakaya (6210881)
Amir Taheri-Ghahfarokhi (6210884)
Elke Ericson (52165)
Mikael Bjursell (274867)
Marta Perez-Alcazar (6210887)
Frank Seeliger (261686)
Magnus Althage (4347592)
Ralph Knöll (799981)
Ryan Hicks (4579402)
Lorenz Mayr (6210893)
Rosie Perkins (3179886)
Daniel Lindén (483184)
Jan Borén (119843)
Mohammad Bohlooly-Y (217638)
Marcello Maresca (5556467)

January 2019

Figure S2. CRISPR-Cas9 targeting strategy and editing efficiency. (a) Schematic of exon 1 of human P...

Additional file 2: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey MiannĂŠ (3407429)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S1. Screening by Sanger sequencing of animals for the generation of a Syt7 conditional allele...

Additional file 1: of Establishment of TP53-knockout canine cells using optimized CRIPSR/Cas9 vector system for canine cancer research

Kiyoung Eun (6173807)
Min Gi Park (6173810)
Yeon Woo Jeong (6173813)
Yeon Ik Jeong (6173816)
Sang-Hwan Hyun (295152)
Woo Suk Hwang (295153)
Sung-Hak Kim (2930070)
Hyunggee Kim (75727)

January 2019

Figure S1. Representative images of isolated canine fibroblasts with an extended cellular life span ...

Additional file 13: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey Mianné (3407417)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S12. Examples of unexpected point mutations in the F0 animals obtained from the co-injection ...

Additional file 2: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Ke Qin (1288881)
Xinyu Liang (487152)
Guanjie Sun (6553118)
Xuan Shi (739853)
Min Wang (21070)
Hongbo Liu (124714)
Yaosheng Chen (134488)
Xiaohong Liu (50816)
Zuyong He (134485)

April 2019

Figure S1. Fluorescent images of porcine kidney cells 24âh after transfection of plasmid pX458-sgR...

Additional file 4: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Ke Qin (1288881)
Xinyu Liang (487152)
Guanjie Sun (6553118)
Xuan Shi (739853)
Min Wang (21070)
Hongbo Liu (124714)
Yaosheng Chen (134488)
Xiaohong Liu (50816)
Zuyong He (134485)

April 2019

Figure S3. Images of cell clones expanded from single porcine kidney cell. One weekâs culture of s...

Additional file 6: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Ke Qin (1288881)
Xinyu Liang (487152)
Guanjie Sun (6553118)
Xuan Shi (739853)
Min Wang (21070)
Hongbo Liu (124714)
Yaosheng Chen (134488)
Xiaohong Liu (50816)
Zuyong He (134485)

April 2019

Figure S5. Further detection of the potential off-target effects of sgRNA17â6. NC indicates the ne...

Additional file 1: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Ke Qin (1288881)
Xinyu Liang (487152)
Guanjie Sun (6553118)
Xuan Shi (739853)
Min Wang (21070)
Hongbo Liu (124714)
Yaosheng Chen (134488)
Xiaohong Liu (50816)
Zuyong He (134485)

April 2019

Table S1. List of sgRNAs designed for targeting the intron16 and intron17 of KIT gene. Table S2. Pri...

Additional file 1: Table S1. of Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs

Jung-Taek Kang (3429896)
Bumrae Cho (304017)
Junghyun Ryu (3429890)
Caitlin Ray (3429908)
Eun-Jin Lee (2299186)
Yun-Jin Yun (3429902)
SunMi Ahn (3429899)
JinSeok Lee (3429905)
Dal-Young Ji (3429887)
Nathaniel Jue (3429884)
Sherrie Clark-Deener (550129)
Kiho Lee (1366734)
Kwang-Wook Park (3429893)

November 2016

Oligos used to introduce sgRNA into px330. Each pair of forward and reverse primers were annealing a...

Additional file 3: Figure S2. of One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system

Methichit Wattanapanitch (627973)
Nattaya Damkham (4889524)
Ponthip Potirat (4889521)
Kongtana Trakarnsanga (597732)
Montira Janan (4889527)
Yaowalak U-pratya (627977)
Pakpoom Kheolamai (627979)
Nuttha Klincumhom (627974)
Surapol Issaragrisil (627981)

February 2018

Showing transfection efficiency of PX458 in the Eβ-iPSC2 cells. (a) Phase contrast and fluorescent i...

Additional file 3: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Figure S1. showing sequencing results of parental and inserted iPSCs. a Parental iPSCs have the know...

Additional file 3: Figure S1. of Deciphering the genetic regulation of peripheral blood transcriptome in pigs through expression genome-wide association study and allele-specific expression analysis

T. Maroilley (4684477)
G. Lemonnier (2370415)
J. Lecardonnel (4684486)
D. Esquerré (4684474)
Y. Ramayo-Caldas (4684483)
M. Mercat (4684492)
C. Rogel-Gaillard (4684480)
J. Estellé (4684489)

December 2017

Distribution of eQTL-SNPs along pig autosomal chromosomes. The histograms represent the distribution...

Additional file 1: of Genome-wide identification of RNA editing in seven porcine tissues by matched DNA and RNA high-throughput sequencing

Yuebo Zhang (595294)
Longchao Zhang (465233)
Jingwei Yue (4439236)
Xia Wei (2063044)
Ligang Wang (347845)
Xin Liu (43569)
Hongmei Gao (240295)
Xinhua Hou (108350)
Fuping Zhao (389140)
Hua Yan (211621)
Lixian Wang (465239)

March 2019

Figure S1. Overview of the experimental design. Figure S2. Results of the Sanger sequencing validati...

Additional file 1: of Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells

Chenzi Zhang (6163493)
Xiangjun He (6163496)
Yvonne Kwok (6163499)
Feng Wang (44414)
Junyi Xue (1533826)
Hui Zhao (7395)
Kin Suen (6163502)
Chi Wang (131921)
Jianwei Ren (438694)
George Chen (179705)
Paul Lai (6163505)
Jiangchao Li (290762)
Yin Xia (307747)
Andrew Chan (352984)
Wai-Yee Chan (18789)
Bo Feng (141991)

December 2018

Figure S1. Cytogenetic analysis of the human cell line LO2. Figure S2. NHEJ-based knock-in of ires-G...

Additional file 1: of Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos

Kayla Carey (6670022)
Junghyun Ryu (3429890)
Kyungjun Uh (6670031)
Andrea Lengi (6670037)
Sherrie Clark-Deener (550129)
Benjamin Corl (6670040)
Kiho Lee (1366734)

May 2019

Table S1. Summary of off-targeting comparison for RAG2. Target gene (RAG2) guide sequence compared t...

Additional file 3: of In vivo genome and base editing of a human PCSK9 knock-in hypercholesterolemic mouse model

Alba Carreras (6210875)
Luna Pane (6163538)
Roberto Nitsch (6210878)
Katja Madeyski-Bengtson (4452094)
Michelle Porritt (5940611)
Pinar Akcakaya (6210881)
Amir Taheri-Ghahfarokhi (6210884)
Elke Ericson (52165)
Mikael Bjursell (274867)
Marta Perez-Alcazar (6210887)
Frank Seeliger (261686)
Magnus Althage (4347592)
Ralph Knöll (799981)
Ryan Hicks (4579402)
Lorenz Mayr (6210893)
Rosie Perkins (3179886)
Daniel Lindén (483184)
Jan Borén (119843)
Mohammad Bohlooly-Y (217638)
Marcello Maresca (5556467)

January 2019

Figure S2. CRISPR-Cas9 targeting strategy and editing efficiency. (a) Schematic of exon 1 of human P...

Additional file 2: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey MiannĂŠ (3407429)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S1. Screening by Sanger sequencing of animals for the generation of a Syt7 conditional allele...

Additional file 1: of Establishment of TP53-knockout canine cells using optimized CRIPSR/Cas9 vector system for canine cancer research

Kiyoung Eun (6173807)
Min Gi Park (6173810)
Yeon Woo Jeong (6173813)
Yeon Ik Jeong (6173816)
Sang-Hwan Hyun (295152)
Woo Suk Hwang (295153)
Sung-Hak Kim (2930070)
Hyunggee Kim (75727)

January 2019

Figure S1. Representative images of isolated canine fibroblasts with an extended cellular life span ...

Additional file 13: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Gemma Codner (3407399)
Joffrey Mianné (3407417)
Adam Caulder (3407402)
Jorik Loeffler (5431574)
Rachel Fell (5431583)
Ruairidh King (5431589)
Alasdair Allan (5431595)
Matthew Mackenzie (5431592)
Fran Pike (5431568)
Christopher McCabe (206680)
Skevoulla Christou (5431577)
Sam Joynson (5431580)
Marie Hutchison (3578627)
Michelle Stewart (618852)
Saumya Kumar (530117)
Michelle Simon (530116)
Loranne Agius (2527249)
Quentin Anstee (5431571)
Kirill Volynski (5431598)
Dimitri Kullmann (5431586)
Sara Wells (106305)
Lydia Teboul (106309)

June 2018

Figure S12. Examples of unexpected point mutations in the F0 animals obtained from the co-injection ...

Additional file 3: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Abstract

Extracted data

Additional file 3: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Abstract

Extracted data

Related items

Related items

Additional file 3: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Abstract

Extracted data

Additional file 3: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Abstract

Extracted data

Related items

Related items

Additional file 3: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Additional file 3: of Highly efficient correction of structural mutations of 450âkb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9