Substitutions in E1B 55K RNP affect viral late mRNA biogenesis.
- Berto Tejera (6551444)
- Raúl E. López (6551447)
- Paloma Hidalgo (6551450)
- Reinier Cárdenas (6551453)
- Grisel Ballesteros (6551456)
- Lina Rivillas (6551459)
- Leidys French (6551462)
- Carlos Amero (153005)
- Nina Pastor (87579)
- Ángel Santiago (6551465)
- Peter Groitl (181762)
- Thomas Dobner (181801)
- Ramón A. Gonzalez (5800742)
Publication date
April 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
HFF cells infected with Ad5 WT or E1B 55K mutants were harvested at 36 hpi and total RNA was isolated. Viral late pre-mRNA levels were determined for L5 RNA by RT qPCR against an (A) intron-exon (L5NP) or (B) Exon-exon (L5P) junction, for the unspliced and spliced L5 mRNA species, respectively. (C) To compare the splicing efficiency the L5P:L5NP ratios were calculated. β actin mRNA was used as endogenous control. Data from two independent experiments performed in triplicate are shown. ** P<0.01, ****P<0.0001.</p
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