Western diet effects on hepatic fatty acid composition.

Livers were extracted for total lipids, saponified and converted to fatty acid methyl esters (FAMES). FAMES were quantified using a gas chromatograph equipped with a flame ionization detector (Materials & methods). Results are expressed as the sum of SFA, MUFA, ω3 & ω6 PUFA, nmoles/mg protein (A), specific fatty acyls (B & C) and the ω3 index, ω6 index & the ratio of ω6 to ω3 PUFA (D). The ω3 and ω6 index represent the fraction of all fatty acids that are either ω3 PUFA or ω6 PUFA. Results in Panel D were normalized to levels in the RD46 group. Results are expressed as mean ± SEM, N = 7–8/group, *, FDR <0.05 versus the RD46 group.