Actin-binding domains mediate the distinct distribution of two <i>Dictyostelium</i> Talins through different affinities to specific subsets of actin filaments during directed cell migration - Fig 7

Behaviors of talin A, talin B, and their actin-binding domains in aspiration assays. (A-F, left) Phase contrast images showing cells aspirated by a micro-pipette (top) and the corresponding time lapse confocal images showing the responses of GFP-myosin II (A), mCherry-actin (B), talin A-GFP (C), GFP-I/LWEQ(talA) (D), GFP-talin B (E), and GFP-I/LWEQ(talB)-PRR-VHP (F) during sucking into the micro-pipette and the subsequent retraction of the aspirated projections. Times are indicated at lower right corners in seconds. The right panels show the time courses of relative fluorescence intensity changes of each GFP fusion protein at the tips of aspirated lobes (diamonds connected by solid lines) and changes in the lobe length (squares connected by broken lines) in a cell of each cell line. The time when the aspirated lobe was most elongated was taken as time zero, and the fluorescent intensities were normalized by their values at time zero. Shading shows the retraction phase. The data of two more cells in each cell line are shown in S4 Fig. Fluorescence intensities of GFP-myosin II, mCherry-actin, talin A-GFP, GFP-I/LWEQ(talA), and GFP-I/LWEQ(talB)-PRR-VHP, but not that of GFP-talin B, increased during the retraction of the lobe. The pair of arrowheads in (A) indicate the edges of the arc-shaped fluorescence signal representing the detached cortex. Scale bar: 5 μm.