Additional file 10: of Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy

Figure S6. Knockdown of PGK1 suppresses autophagy-associated chemoresistance of gallbladder cancer cells in vitro. (A) The protein levels of PGK1 in Dox-resistant gallbladder cancer cells under different transfection were determined by western blot assay. (B) The sensitivities of GBC-SD/Dox cells under different transfection with Dox were determined by CCK-8 assay. (C) The protein levels of LC3 in GBC-SD/Dox cells under different transfection with CQ (10 μM) were determined by western blot assay. (D) The protein levels of PGK1 in Dox-resistant gallbladder cancer cells under different transfection were determined by western blot assay. (E) The sensitivities of GBC-SD/Dox cells under different transfection with Dox were determined by CCK-8 assay. (F) Relative expression of GBCDRlnc1 in mouse tumor tissues under different transfection with Dox was determined by qRT-PCR. The mean ± SD of triplicate experiments were plotted, ***P < 0.001. (TIF 4358 kb