Peripheral T cells (n = 3, 3 independent experiments) were incubated with FL-OPN, Thr-cOPN or MMP-cOPN under serum free conditions for 72 h. Release of ATP was assessed and ascribed to viable cells. Data was calculated by normalizing to the values without OPN (negative control). Complete medium included 10% FCS; mean ± SEM depicted; Comparison of OPN treated groups with the respective BSA control; * p < 0.05, ** p < 0.01.</p
(A) PBT were cultured for 7 days with 500nM of AS1842856 or vehicle only and their cell size (FSC) a...
<p>Monocytes were incubated overnight in the absence or presence of unstimulated or soluble αCD3 (0....
<p><b>Copyright information:</b></p><p>Taken from "Molecular mechanisms of extracellular adenine nuc...
(A) Jurkat cells (2 experiments, each run in duplicate) and (B) primary T cells (4 experiments utili...
(A-C) Blockade of the interaction of primary human T cells with OPN with monoclonal antibodies 21–5 ...
<p>(A) FACS analysis of monocytes stained for CD14 24 hours after culture in GM-CSF (top panel) or G...
<p>A, PHA- stimulated PBLs were incubated with 33 nM FK866 and <i>ΔΨ</i><sub>m</sub> was determined ...
Ntrations of ATP. Next, cells were extensively washed and co-cultured for 3 days with heterologous C...
<p>P14 CD44<sup>lo</sup>CD8<sup>+</sup> T<sub>N</sub> were stimulated for 6 days <i>in vitro</i> wit...
<p>PBMC were treated or not overnight with 10 ng/mL LPS, washed and then treated simultaneously or n...
Polyclonal stimuli like phorbol myristate acetate (PMA) plus calcium ionophore (Ca-I), concanavalin ...
<p>A, PHA-stimulated PBLs were treated (or not) with 33 nM FK866 in the presence or absence of 10 mM...
<p>Spleen cells from 3 to 4-mo-old MRL<i><sup>+/+</sup></i> (<i>solid line</i>), MRL/<i>lpr</i> (<i>...
<p>PBMC were first treated with medium (white points) or primed with 10 ng/mL LPS (black points), th...
<p>PBMC were primed overnight with LPS concentrations ranging from 0.01 ng/mL to 100 ng/mL (in case ...
(A) PBT were cultured for 7 days with 500nM of AS1842856 or vehicle only and their cell size (FSC) a...
<p>Monocytes were incubated overnight in the absence or presence of unstimulated or soluble αCD3 (0....
<p><b>Copyright information:</b></p><p>Taken from "Molecular mechanisms of extracellular adenine nuc...
(A) Jurkat cells (2 experiments, each run in duplicate) and (B) primary T cells (4 experiments utili...
(A-C) Blockade of the interaction of primary human T cells with OPN with monoclonal antibodies 21–5 ...
<p>(A) FACS analysis of monocytes stained for CD14 24 hours after culture in GM-CSF (top panel) or G...
<p>A, PHA- stimulated PBLs were incubated with 33 nM FK866 and <i>ΔΨ</i><sub>m</sub> was determined ...
Ntrations of ATP. Next, cells were extensively washed and co-cultured for 3 days with heterologous C...
<p>P14 CD44<sup>lo</sup>CD8<sup>+</sup> T<sub>N</sub> were stimulated for 6 days <i>in vitro</i> wit...
<p>PBMC were treated or not overnight with 10 ng/mL LPS, washed and then treated simultaneously or n...
Polyclonal stimuli like phorbol myristate acetate (PMA) plus calcium ionophore (Ca-I), concanavalin ...
<p>A, PHA-stimulated PBLs were treated (or not) with 33 nM FK866 in the presence or absence of 10 mM...
<p>Spleen cells from 3 to 4-mo-old MRL<i><sup>+/+</sup></i> (<i>solid line</i>), MRL/<i>lpr</i> (<i>...
<p>PBMC were first treated with medium (white points) or primed with 10 ng/mL LPS (black points), th...
<p>PBMC were primed overnight with LPS concentrations ranging from 0.01 ng/mL to 100 ng/mL (in case ...
(A) PBT were cultured for 7 days with 500nM of AS1842856 or vehicle only and their cell size (FSC) a...
<p>Monocytes were incubated overnight in the absence or presence of unstimulated or soluble αCD3 (0....
<p><b>Copyright information:</b></p><p>Taken from "Molecular mechanisms of extracellular adenine nuc...