Additional file 6: of The 1H NMR serum metabolomics response to a two meal challenge: a cross-over dietary intervention study in healthy human volunteers

Permutation analysis of ANOVA-PLS, showing actual model misclassifications as vertical lines with p-value calculated as the cumulative probability of finding the actual model result is a Student’s t-distribution of misclassification results obtained from randomly permuted data (n = 300 per model). The permutation distribution had median value of 91 misclassifications, corresponding exactly to the expected value of 182 observations in a two-class problem. The results show strong predictive modelling performance with absence of overfitting in the validation frameworks. (EPS 418 kb